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Partner name:
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Deutsches Krebsforschungszentrum
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Short name:
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DKFZ
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Country:
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Germany
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Role:
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CR (contractor)
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The DKFZ has carried out systematic screens with expression profiling in a number
of cancers, including renal clear cell carcinoma, breast cancer, brain tumors, and
GIST. In previous work within the German cDNA Consortium the partner has identified
and cloned in a GATEWAY-compatible manner a set of 800 full-length open reading
frames coding for proteins with unknown function. Of these 400 have already been
pre-characterized with regard to the subcellular localization of the encoded proteins
and 500 have been cloned for native protein expression. High-throughput assays for
cell proliferation (bromodeoxyuridine incorporation) and apoptosis (Caspase-3 cleavage)
are already operative. Databases for management and integration of information with
data obtained from expression profiling and protein arrays have been set up. Tools
for the web-presentation of this data are under development (www.dkfz.de/LIFEdb).
Given its expertise, the DKFZ will coordinate the workpackage 2, which is aimed
at producing and delivering full-length verified ORFs.
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Name
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Title
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Sex
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Relevant Expertise
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Annemarie Poustka
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PROF
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F
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Head of Division
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Peter Lichter
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PROF
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M
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Head of Division
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Holger Sültmann
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DR
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M
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RNA expression profiling
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Stefan Wiemann
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DR
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M
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cDNA based technologies
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Dorit Arlt
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DR
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F
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cell-based assays
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Lee Bergman
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DR
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F
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cell-based assays
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Stefanie Bechtel
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DR
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F
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cDNA resources
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Boris Zielinski
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DR
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M
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CGH and matrix CGH
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- Wiemann et al., 2001. Toward a Catalog of Human Genes and Proteins: Sequencing and
Analysis of 500 Novel Complete Protein Coding Human cDNAs. Genome Res 11, 422-435.
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Wessendorf et al., 2002. Automated screening for genomic imbalances using matrix-based
comparative genomic hybridization. Lab Invest 82(1):47-60
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Mollenhauer et al., 1997. DMBT1, a new member of the SRCR superfamily, on chromosome
10q25.3-q26.1 is deleted in malignant brain tumours. Nature Genet. 17, 32-39 (1997).
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Fritz et al., 2002. Microarray-based copy number and expression profiling in dedifferentiated
and pleomorphic liposarcoma. Cancer Res 2002 Jun 1;62(11):2993-8
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Simpson, J. C., Wellenreuther, R., Poustka, A., Pepperkok, R., and Wiemann, S. Systematic
subcellular localization of novel proteins identified by large scale cDNA sequencing.
EMBO Rep 1, 287-292 (2000).
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UPCOMING EVENT
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OECI week |
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OECI Week (WG workshops; Scientific Conference and General Assembly) –
Budapest, 16 to 18 June 2010.
read more..
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